IL-3 does not contribute to induction of CML-like disease by

BCR/ABL. Kaplan-Meier–style survival curve for recipients of marrow transduced with p210 BCR/ABL. The genotypes of donor and recipient are indicated by the dashed lines; all mice were of Balb/c background. The number of recipients in each arm is shown in parentheses. All recipients developed typical CML-like leukemia (see text). Omission of recombinant IL-3 from the prestimulation and transduction medium had no effect on leukemogenesis (data not shown). The curve with wild-type donors and recipients is a composite of previously reported mice6 and additional mice transplanted concurrently with the Il-3 andGm-csf mutant mice. There were no significant differences in survival between wild-type (WT) recipients of transduced WT marrow and transplants where either donor or recipient wasIl-3^−/− (P = .65, Mantel-Cox test), whereas the survival of Il-3^−/− mice transplanted with Il-3^−/− marrow was significantly shorter (P = .01).