Cell cycle analysis and annexin staining of apoptotic cells from BCR/ABL-BaF3 cells grown in SCH66336.

(A) BCR/ABL-BaF3 cells grown in medium containing 0, 1, or 10 μM SCH66336 were subjected to cell cycle analysis. Fixed cells were stained with propidium iodide and analyzed by flow cytometry. Increased concentrations of SCH66336 caused a reduction in the number of cells in S phase and an increase in G2/M. (B) BCR/ABL-BaF3 cells grown in medium containing 0, 1, or 10 μM were stained with propidium iodide to identify dead cells and annexin antibody to detect cells that had initiated apoptosis. Increasing concentrations of SCH66336 caused a modest increase in the number of cells that initiated apoptosis, as seen in the annexin-positive, propidium iodide–negative population (lower-right quadrant).