Methylcellulose colony assay of normal and CML human primary cells grown in SCH66336.

Normal bone marrow (n = 3) and CML bone marrow or peripheral blood cells (n = 2) were grown in methylcellulose containing the indicated concentrations of SCH66336. Colony formation in the CML samples was significantly reduced by SCH66336, with an IC₅₀ between 500 and 1000 nM. Normal samples were significantly less sensitive to SCH66336 colony inhibition. Colony counts were assessed on each individual sample at least twice, and results are presented as the average ± SD for colonies counted from duplicate plates under each condition.