c-MET and HGF expression in Hodgkin disease.

(A,B) HD, NS subtype in an immunocompetent patient. Immunohistochemical detection of c-MET with the C-28 rabbit pAb (A) and competitive inhibition with molar excess of blocking peptide relative to C-28 (SC-161) (B). (C) HD, NS subtype in an immunocompetent patient. A frozen section was tested by 2-color staining with the C-28 rabbit pAb and antihuman HGF goat antiserum. A c-MET⁺ RS cell showing strong cytoplasmic and nuclear staining in red is surrounded by several HGF⁺ cells (cytoplasmic reaction in brown). (D) Double-labeled immunofluorescence showing detection of c-MET (red fluorescence) and CD30 mAb (green fluorescence). CD30⁺ RS cells exhibit membranous and cytoplasmic c-MET immunoreactivity. Yellow areas indicate colocalized CD30 and c-MET immunoreactivity. (E) Double-labeled immunofluorescence showing detection of c-MET (red fluorescence) and α5β1 integrin (green fluorescence). C-MET⁺ RS cell shows a membranous α5β1 immunoreactivity. In the same field a c-MET⁻ stromal cell displays a weak α5β1 expression. (F) Double-labeled immunofluorescent detection of HGF (red fluorescence) and CD21 (green fluorescence). In a cluster of CD21⁺ dendritic-reticulum cells, one element exhibits both CD21 and cytoplasmic HGF immunoreactivity. ABC-px immunostaining with hematoxylin counterstaining is shown in panels A and B (original magnification × 100), and the immunostaining in panels C-F is described above (original magnification × 630).