MHC class I expression in K14-β2m mice.

(A) Lack of H-2K^b expression on K14-β2m splenic B cells, T cells, macrophages, and dendritic cells. Splenocytes from K14-β2m mice (black lines) and β2m^+/° littermates (gray lines) were stained with anti–H-2K^b combined with anti-B220, anti-TCRβ, anti–Mac-1, or anti-CD11c antibodies. Histograms of H-2K^b expression on electronically gated B220⁺ (B cells), TCRβ⁺ (T cells), Mac-1⁺ (macrophages), or CD11c⁺ (dendritic cells) subsets are representative of 3 independent experiments. (B) Thymic cortical epithelial expression of MHC class I in K14-β2m mice. Sections of wild-type C57BL/6, C57BL/6-β2m°, and K14-β2m thymi were doubly stained with FITC-conjugated antibody 6C3, which detects cortical epithelial cells, and rhodamine-labeled H-2K^b– and H-2D^b–specific antibody 28-8-6. Green and red fluorescence of the same fields is shown.