CD8⁺ T lymphocytes from K14-β2m mice kill syngeneic MHC class I⁺ hematopoietic cells in vivo.

Lethally irradiated C57BL/6 hosts were reconstituted with a mixture of C57BL/6 and C57BL/6-β2m° bone marrow cells that were cotransferred with C57BL/6 or K14-β2m lymph node (LN) cells. Where indicated, the K14-β2m lymph node cells were depleted of either CD4⁺ (LN ΔCD4) or CD4⁺ plus CD8⁺ (LN ΔCD4ΔCD8) cells (by antibody plus complement treatment) before transfer. Reconstitution by C57BL/6 bone marrow cells was assessed 2 weeks later by 2-color flow cytometry of bone marrow with the use of anti-CD43 (detecting B cell precursors) and anti–H-2K^bantibodies.