The donor CTL line demonstrated specificity only for 2 epitopes in EBNA-3B.

(A) This figure shows the cytotoxic activity of the donor CTL line against HLA-matched CA (HLA-B60) or CR (HLA-A2, -A11, and -B7) OKT3 blasts pulsed with 10⁻⁵ mM peptide at an effector:target ratio of 5:1. Peptide coordinates are shown. E2 represents EBNA-2, and L2 represents LMP2. C represents killing of OKT3 blasts in the absence of added peptide. Peptides that have been shown to sensitize target cells to killing through HLA-A2, -A11, -B7, and -B60 as well as control B44-restricted peptides are described in Table 1. (B) The donor CTL line was specific for peptides in EBNA-3B. The peptides that looked active in sensitizing CR OKT3 blasts at 10⁻⁵ M were used in the same assay at dilutions from 10⁻⁵ to 10⁻¹¹ M. Only AVFDRKSDAK and IVTDFSVIK, the peptides deleted from EBNA-3B, titrated out to 10⁻⁸ M.indicates 42-51 (E2); ○, 379-387 (E3A); ▴, 399-408 (E3B); ▪, 416-424 (E3B); ●, 551-563 (E3B); and ░, 281-290 (E3C).