TCR BV spectratyping results for 3 BV gene family members from a single patient.

Full BV spectratypes were generated for preculture, postculture unselected, and postculture CD8⁺ selected samples. In most cases, postculture spectratype appearances were similar to preculture appearances, as is shown for BV13 and BV3 (in duplex PCR reactions). Some spectratypes had one or more dominant size-class peaks after culture and this was often more pronounced after selection for cells bearing CD8 and with increasing culture duration, as is shown for BV6.1 after 2 and 3 weeks of culture.