TG5 mice developed an enhanced and sustained eosinophilic inflammation following thioglycolate injection.

(A) Total cell numbers of peritoneal lavage samples were assessed using Coulter counter before and 1, 6, 12, 24, 48, and 72 hours after thioglycolate injection as described. Counts per milliliter were adjusted to the volume recovered in each animal. (B) Absolute eos cell numbers in peritoneal lavage fluid were calculated using the number of total cells (as above) and the percentage of eos assessed from cytospin preparations in each animal. (C) Cytospin preparations from bone marrow samples were analyzed for eos cell counts before and 1, 6, 12, 24, 48, and 72 hours after thioglycolate injection. (D) Peripheral blood smears from the tail vein were analyzed for eos cell counts before and 24, 48, and 72 hours after thioglycolate injection. Differential cell counts were made by counting at least 200 cells in each sample. Results are expressed as percentage of total counted cells. For each study values are expressed as mean ± SEM (n = 4 samples were studied for each time point and each genotype). Open circles indicate cell numbers in control (FVB) mice; solid circles, cell numbers in transgenic mice TG5.