MBP release in the peritoneal cavity in TG5 and FVB mice.

Representative Western blots of MBP content analysis from peritoneal lavage supernatant samples. Western blots were performed using a rabbit polyclonal antiserum developed in our laboratory as described. Each panel shows protein of expected molecular weight (14 kd) 1, 6, 12, 24, 48, and 72 hours after thioglycolate injection. Eosinophilic cell lysates derived from IL-9 transgenic bone marrow preparations were used as positive controls. Splenic cell lysate preparations were used as negative control.