Eotaxin expression in TG5 and FVB mice.

(A) Representative Northern blots showing eotaxin mRNA production in mesenteric lymph nodes from TG5 (T) and FVB (F) mice. Mesenteric lymph nodes were harvested before and 1, 12, and 72 hours after thioglycolate injection. Hybridization with a GAPDH probe confirmed that comparable amounts of RNA had been loaded in each lane. (B) Time course of immunoreactive eotaxin protein release in the peritoneal lavage was analyzed using ELISA. Supernate samples were harvested before and 1, 6, 12, 24, 48, and 72 hours after thioglycolate injection, respectively. Values are expressed in pg/mL as mean ± SEM (n = 4 for each time point and each genotype). Open circles indicate cell numbers in FVB mice; solid circles, cell numbers in TG5 mice.