American Society of Hematology

Fig. 1.

$Fig. 1. Expression of DMT1 protein in the duodenum of. / Trf hpx/hpx,Hfe−/−, andB2m−/− mice. Membrane proteins isolated from proximal duodenum of the different mice were fractionated on 10% acrylamide gels and transferred to polyvinylidene difluoride membranes. For comparison, microsomal fractions from mk/+and mk/mk mice were included in the analysis. To demonstrate the specificity of the anti-DMT1 antibody, membrane proteins from CHO cells or CHO cells expressing a c-Myc–tagged DMT1 protein (CHO-DMT1) were also included (Ai and Aii). Immunoblotting was performed with antibodies raised against DMT1 (Ai and ii), transferrin receptor (Bi and Bii), and Bgp1 proteins (Ci and ii). The positions and sizes (in kd) of molecular weight markers are indicated on the right.$

Expression of DMT1 protein in the duodenum of

Trf^hpx/hpx,Hfe^−/−, andB2m^−/− mice. Membrane proteins isolated from proximal duodenum of the different mice were fractionated on 10% acrylamide gels and transferred to polyvinylidene difluoride membranes. For comparison, microsomal fractions from mk/+and mk/mk mice were included in the analysis. To demonstrate the specificity of the anti-DMT1 antibody, membrane proteins from CHO cells or CHO cells expressing a c-Myc–tagged DMT1 protein (CHO-DMT1) were also included (Ai and Aii). Immunoblotting was performed with antibodies raised against DMT1 (Ai and ii), transferrin receptor (Bi and Bii), and Bgp1 proteins (Ci and ii). The positions and sizes (in kd) of molecular weight markers are indicated on the right.

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