Influence of route of administration on rAAV-mediated hFIX expression in vivo.

rAAV CAGG-FIX vector particles (5 × 1010) were administered into the portal vein (solid bars), tail vein (checkered bars), or muscle (shaded bars) in 10-week-old male C57Bl/6 SCID mice. Blood samples were obtained at the indicated time point after rAAV administration, and the hFIX concentration in murine plasma was determined by an ELISA. Each bar represents an average of 4 animals with SEM.