Fig. 3.
Pulse-chase of cell lysate and conditioned media of the cells stably transfected with pEDVIIWT or pEDVIIMT.
After 15 minutes of pulse with 35S-methionine, cells were chased for 30, 60, 120, and 240 minutes. Equivalent amounts of (A) cell lysate and (B) conditioned media for both WT and MT constructs were immunoprecipitated using mAb1476 against VII and analyzed by 8% SDS-PAGE under nonreducing conditions. The molecular weight marker is localized at the right side of the figures. The difference in electrophoretic mobility is shown by arrows.
