Fig. 1.
Effect of neutrophil culture on plasminogen-binding capacity.
Freshly isolated human blood neutrophils (1.5 × 107), defined as time 0, were cultured for the indicated times in Mo-Medium at 37°C in a humidified atmosphere, 5% CO2, with 5% FBS or varying amounts of FBS. After washing the cells, specific plasminogen binding was quantitated by means of 125I-plasminogen at 100 nM and a 1-hour incubation. (A) Cultured with 5% FBS. This time-course experiment (means ± SEM of triplicates) is representative of 3 experiments. (B) Cultured with varying amounts of FBS. This FBS dose-titration experiment was done twice.
