Effect of CpB treatment on the up-regulation of plasminogen binding.

Neutrophils were cultured as in Figure 1A for 20 hours. After washing, the cells were treated with 10 (50 μg/mL) and 50 U/mL (250 μg/mL) of CpB for 1 hour at 37°C in the presence or absence of the CpB inhibitor GEMSA (G) at 0.3 mM. Plasminogen binding was then measured. The controls are freshly isolated neutrophils (after isolation) or the untreated, cultured neutrophils. The experiment shown (means ± SEM of the triplicates) is representative of 3 different experiments.