Effect of protease inhibitors on the up-regulation of plasminogen binding induced by PMA.

Neutrophils (1 × 10⁷) were stimulated with 0.1 and 0.5 nM PMA for 20 minutes at 37°C in the presence or absence of SBTI (100 μg/mL) or HLE/CMK (10 μM). After washing, specific plasminogen binding was quantified. The results are from one experiment (means ± SEM of the triplicates) and are representative of at least 3 such experiments. In a set of 6 other experiments, the cathepsin G inhibitor CG/CMK (2 to 25 μM) did not inhibit the PMA-induced increase in plasminogen receptor expression (results not shown).