FACS analysis of neutrophils from blood of control mice (dashed lines) and from the peritoneal cavity of mice challenged with thioglycollate (solid lines).

Inclusion of EACA in the incubation media decreased plasminogen binding to both the peripheral blood and peritoneal neutrophils by a similar amount. (A) Neutrophils were incubated in buffer followed by FACScan analysis (negative control). (B) Neutrophils were incubated with Alexa Fluor 488–labeled plasminogen for 1 hour at 37°C before FACS.