SU5416-treated AML blast cells demonstrate increased cleavage of PARP.

AML blasts (patient No. 1) in medium containing 10% FBS were pretreated with SU5416 for 1 hour prior to incubation with 100 ng/mL SCF. At 24 hours, cells were lysed and proteins separated by SDS-PAGE. After transfer to nitrocellulose, the blots were probed with an anti-PARP antibody. The full-length 116-kd PARP and the 26-kd cleaved PARP fragment are indicated.