Inhibition of RBC Cl-conductance after IV and PO administration of NS3623 to normal mice.

Animals were killed and bled at various times after dosing, and the packed blood cells were transferred to buffer-free salt solutions containing CCCP for recording of valinomycin-induced hyperpolarizations. Each point is a mean of measurements on RBCs from 3 mice. Error bars represent SD. (A) Time dependence of the Cl-conductance block after IV administration (at t = 0) of NS3623 (50 mg/kg). (■) indicates uninjected; (○), vehicle-injected (t = 1 minute); and (●), NS3623-injected. (B) Dose-dependence (5-150 mg/kg) of the Cl-conductance block recorded 1 hour after PO administration of NS3623. The dashed line shows the hyperpolarization at normal chloride conductance (mean of control hyperpolarizations).