Time-course of Taxol- and epirubicin-induced processing of caspase-3 and caspase-8 in BJAB cells.

BJAB cells were cultured in control medium (Co), 0.1 μg/mL Taxol (T), or 1 μg/mL epirubicin (E) for different time intervals as indicated. Western blot analyses were performed as described in “Materials and methods” with antihuman caspase-3 (A) and antihuman caspase-8 antibodies (B). Positions of the molecular mass markers are indicated at the left. Arrows indicate the positions of procaspase-3 (*), the 17-kd active subunit of caspase-3 (**), procaspase-8 (#), and the 18-kd active subunit of caspase-8 (##). Experiments were repeated twice and yielded similar results.