Northern blot analysis of CD95/Fas ligand expression in epirubicin-induced cells.

Leukemic cell samples of 5 patients (the same as in Table 1) with childhood B-lineage ALL (A) were induced for 24 hours in the presence of epirubicin (0.1 μg/mL) or medium alone. BJAB (B) or REH cells (C) were induced for 24 to 72 hours in the presence of epirubicin. M, medium control at 72 hours. (D) As a positive control for the induction of CD95/Fas ligand expression, Jurkat T cells were cultured in the presence of plastic-immobilized anti-CD3 monoclonal antibody OKT3 for 0, 1, 2, or 4 hours. To control for equal amounts and the integrity of the RNA, the blots were rehybridized with a cDNA probe for β-actin.