Effects of blocking of CD95 ligand–CD95 receptor interaction.
BJAB (A) or REH (B) B cells were induced for apoptosis by epirubicin (0.1 μg/mL) in the presence or absence of neutralizing anti-CD95/Fas or control F(ab)2 fragments. Open circles, medium control; open triangles, epirubicin; open squares, epirubicin plus control F(ab)2; filled squares, epirubicin plus anti-CD95/Fas F(ab)2 fragments. Jurkat T-cells (C) were induced for apoptosis for 24 hours in the presence (squares) or absence (circles) of immobilized anti-CD3 as described.19 Control (open circles, open squares) or anti-CD95/Fas F(ab)2 fragments (filled circles, filled squares) were present in the cultures at 1 μg/mL. Apoptosis was measured at the single cell level by assessing the nuclear DNA content as described in “Materials and methods.” Mean values for the percentage of apoptotic cells ± SD (n = 3) are shown.
