BJAB cells overexpressing a dominant-negative FADD mutant (FADDdn) are resistant to CD95-mediated apoptosis.

(A) Expression of FADDdn. Lysates of vector-transfected BJAB cells and BJAB cells stably expressing FADDdn were subjected to immunoblotting using a monoclonal anti-FADD antibody. The positions of molecular mass markers are indicated at the left. (B) Apoptosis induction. BJAB vector control or FADDdn-expressing cells were either left untreated (Control) or were stimulated for 30 hours with 1 μg/mL anti-CD95 antibody. Then cell death was assessed by the uptake of propidium iodide and flow cytometry as described in “Materials and methods.” Values are given as percentage of dead cells ± SD (n = 3).