Flow cytometry.

Flow cytometric analysis of bone marrow collected from a representative nonmyeloablated MPS VII neonatal transplant recipient of adult bone marrow at 1 year after engraftment (first column). Marrow was also collected from an untreated MPS VII control for comparison (second column). RBCs were removed by lysis and nucleated cells were incubated with the fluoresceinated GUS substrate Imagene Green FDGlcU followed by staining with phycoerythrin (PE)-conjugated lineage marker antibodies. These antibodies included Mac-1 (CD11b), Gr-1, B220 (CD45R), or αβTcR for macrophages, granulocytes, B cells, and T cells (αβ receptor), respectively. All transplant recipients showed a similar pattern of GUS⁺, lineage-positive cells demonstrating multilineage engraftment by +/+ donor HSC.