Fig. 1.
Expression of MDM2 and p53 protein in BL2, Saos-2, Priess, and Ramos cell lines.
Equal quantities of protein extracts obtained from the indicated cell lines were separated on 7.5% SDS-PAGE, transferred to PVDF membranes, and probed with either an anti-MDM2 mAb (IF2) or anti-p53 mAb (DO1). After incubation with a rabbit antimouse IgG, the blots were revealed with [125I]-protein A and submitted to autoradiography.