Fig. 1.
mDYRK-3 transcripts are expressed at high levels in erythroid cell lines.
In a panel of murine cell lines, levels of mDYRK-3 transcript expression were assayed by Northern blotting. Included were Friend-virus–transformed MEL and SKT6 erythroleukemic cells, Epo-responsive and εy-globin positive B6SUt.EP cells, lymphoid CTLL-2(ER) cells, myeloid FDC(ER) cells, and NIH 3T3 fibroblasts. Also analyzed were splenocytes from control and phenylhydrazine-treated mice. Probes used were 32P-labeled 5′ fragments of human DYRK-3 and murine GATA-1 cDNAs. Equivalence in loading was assessed by hybridization to a32P-GAPDh cDNA.