Expression of mDYRK-3, mDYRK-2, and mDYRK-1a among murine tissues and within control splenocytes, erythroid splenocytes, and brain.

(A) Transcript-specific probes were prepared by RT-PCR, cloned to pCRScript, ³²P-labeled, and hybridized to an array of polyA⁺ RNAs from murine tissues. Graphed are relative levels of transcript expression, with values normalized to levels in testes. (B) ³²P–RT-PCR was used to assay levels of mDYRK-3, mDYRK-2, and mDYRK-1a transcripts in brain, control splenocytes (“spleen”), and erythroid splenocytes from mice treated with TAP. As an internal control, hgprt transcripts were also assayed. At the cycles indexed, products were analyzed by polyacrylamide gel electrophoresis and autoradiography.