Deactivation of chemotaxis and directed migration induced by AT III or Kybernin P.

Neutrophils were pretreated for 20 minutes with Kybernin P or AT III before migration to fMLP or IL-8 was tested. Homologous deactivation by IL-8 served as a positive control (A,B). Time-course of pretreatment for AT III– or IL-8–induced deactivation of neutrophil chemotaxis to IL-8 (C). Directed migration of neutrophils to Kybernin P or AT III (D). Migration time into nitrocellulose filters was 30 minutes. After fixing and staining of filters, migration depth was quantified microscopically. The chemotaxis index is the ratio between the distances of directed and undirected migration of neutrophils. Means of chemotaxis indices were compared by the Mann-Whitney test after Kruskal-Wallis analysis of variance (P < .001). *P < .05; **P < .01. n = 13 (A,B); n = 5 (C); n = 9 (D).