Effects of AT IIIED or TAT complex on neutrophil migration.
Untreated neutrophils migrated to various concentrations of AT IIIED (○) or TAT complexes (●). AT III and IL-8 served as positive controls (left). For deactivation experiments, cells were pretreated with either AT IIIED or TAT complexes for 20 minutes before chemotaxis to IL-8 was run. Preincubation with AT III was the positive control (right). Cells migrated for 30 minutes into nitrocellulose filters, and, after fixing and staining of filters, migration depth was quantified microscopically. The chemotaxis index is the ratio between the distances of directed and undirected migration of neutrophils. Means of chemotaxis indices were compared by Mann-Whitney test after Kruskal-Wallis analysis of variance (P < .001). **P < .01. n = 5.
