Fig. 6.
Role of S residues of STAT5 for initiation of Py replication.
(A) Various amounts of GAL4–STAT5B-wild or GAL4–STAT5B-6XSA were transfected to BA/F-wild cells together with SRα-LTag and pPyG5OICAT. (B) Two micrograms of GAL4–STAT5B or GAL4–STAT5B S-residue mutants were transfected with SRα-LTag and pPyG5OICAT. Cells were cultured for 24 hours in the presence of hGM-CSF (10 ng/mL). Replication was analyzed by DpnI assay. The arrows indicate replicated plasmid.