SDF-1 and MDC induce bleb formation in platelets and cause microaggregation.

Washed platelets were subjected to SDF-1 or MDC at 0.5 μg/mL or to ADP under low-shear stresses for 10 seconds at 37°C, as described for Figure 2, and then fixed with glutaraldehyde for subsequent processing and examination by scanning electron microscopy.25 (A) Control. (B) Low ADP level (0.05 μM). (C) High ADP level (10 μM). (D) SDF-1. (E) MDC. (F) MDC–SDF-1 combination. (G) Low ADP and SDF-1. (H) Low ADP and MDC. (i) MDC. The white bar indicates 1 μm. The micrographs are representative of results with platelet preparations from 3 donors.