Effect of c-RGD (10 μg/mL) and anti-α_vβ₃-antibody (LM609, 10 μg/mL) on the adherence to and invasion in fibrin matrices by hMVEC.

(A) Cells were allowed to adhere to vitronectin in the presence or absence of LM609 (10 μg/mL) or to fibrin in the presence c-RGD (10 μg/mL), anti-FITC mAb (10 μg/mL) (control Ab), and LM609 (10 μg/mL). Cells were also allowed to adhere to a matrix prepared from fibrinogen_Nieuwegein. After 2 hours the adhered cells were counted and expressed as percent of control (no addition). Results from 6 cultures in 3 independent experiments are given and expressed as mean ± SD. (B) The hMVEC were seeded on top of the fibrin matrices prepared from normal and fibrinogen_Nieuwegein and were stimulated to invade by 5 ng/mL bFGF and 1 ng/mL TNF-α alone or in the presence of c-RGD (10 μg/mL), anti-FITC (10 μg/mL), or LM609 (10 μg/mL). After 6 days the formed tube length was measured and expressed as percent of control (bFGF/TNF-α without addition of antibodies). Data are the mean ± SD of 6 cultures in 3 independent experiments. (*representsP < 0.05)