Biochemical analysis of p140 molecules.

The NK clone AM61 (p140+/p70−), derived from a healthy donor, and the cell lines Pno and Cou-L were surface labeled with biotin and immunoprecipitated with anti-p140 mAb. Samples were treated (+) or not (−) with N-glycosidase F and analyzed in 8% SDS-PAGE under reducing conditions. Molecular weight markers (kd) are indicated on the right.