Impact of NO on CoCl₂, DFX, or hypoxia-induced HIF-1α accumulation.

LLC-PK₁ cells were stimulated with vehicle, 100 μM (A,C,E) or 1 mM (B,D) GSNO, 100 μM CoCl₂ (A,B), 250 μM DFX (C,D), or hypoxia (E), either alone or in different combinations. Cells were lysed and prepared for HIF-1α Western blot analysis after times indicated. Results are representative for at least 5 independent experiments.