Fig. 5.
Overexpression of iNOS promotes HIF-1α accumulation.
LLC-PK1 cells were transfected with the pCIS plasmid containing the human iNOS sequence (pCIS/hiNOS) or a control plasmid as described in “Materials and methods.” Twenty-eight hours after transfection, cells were prepared for Western blots to analyze HIF-1α accumulation (A) and iNOS expression (B). For control reasons, untransfected LLC-PK1 cells were exposed to vehicle (control) or 250 μM DFX for 4 hours. Blots are representative for 3 independent experiments.
