Expression of cdc2 and cell cycle arrest of T lymphocytes after MTD.

Scatter density diagrams of bivariate (cdc2/DNA) cell cycle analysis of activated T lymphocytes from wild-type mice (p21^+/+; A,C) and p21 knockout mice (p21^−/−; B,D) are shown. After activation, cells were treated for 24 hours with 15 μg/mL nocodazole (C,D) or control diluent (A,B). 2N (G₀/G1) and 4N (G2/M) DNA content is indicated along with the percentages of cells in different cell cycle phases. Data are representative of at least 2 replicate experiments from 6 sets of mutant mice and their wild-type littermate controls.