Effect of cycloheximide on HO-1 mRNA induction by ZnPP.

HA-1 cells were preincubated with various doses of cycloheximide for 15 minutes prior to incubation with 10 μM ZnPP. HO-1 mRNA was evaluated by Northern analysis and normalized to the β-actin mRNA obtained from the same blot. Lane Cyc, cells incubated with 2 mM cycloheximide alone for 15 minutes as control; lane ZP, cells incubated with 10 μM ZnPP for 24 hours. Lanes 0.05, 0.1, 0.3, 1, and 2 show cells preincubated with 0.05, 0.1, 0.3, 1, or 2 mM cycloheximide and then incubated with ZnPP for 24 hours.