Markers of apoptosis in MP-incubated cells.
(A) A representative slide of immunoreactive annexin V detection in cultured HA-1 cells incubated with 10 μM MPs for 24 hours. Four slides in each group were incubated with annexin V antibody and FITC as described in “Materials and methods.” All images were obtained at the same intensity to allow for comparison. Lane C, untreated controls; lane CP, cells incubated with CrPP; lane SP, cells incubated with SnPP; lane ZP, cells incubated with ZnPP. (B) Upper panel shows representative example of 2 Western analyses for immunoreactive p53 protein content in HA-1 cells after 10 μM MP incubation. Lane p53, p53 protein standard obtained from human 293 cells; lane C, untreated controls; lane ZC, cells incubated with 100 μM ZnCl2; lane ZP, cells incubated with ZnPP;lane SP, cells incubated with SnPP; lane CP, cells incubated with CrPP. Lower panel indicates that p53 protein induction was dose dependent on ZnPP concentration. Lane p53, p53 protein standard obtained from human 293 cells; lane C, untreated controls; lane 1, cells incubated with 1 μM ZnPP; lane 10, cells incubated with 10 μM ZnPP; lane 50, cells incubated with 50 μM ZnPP.
