Fig. 2.
Effects of adhesion.
Comparison of the effects of adhesion to FN peptides on proliferation of G1E-ER2 cells (A) and primary EPCs (B). Cells were cultured on FN-peptide–coated dishes mediating adhesion via both α4β1 and α5β1(CH296) or α4β1 (H296) or α5β1 (CH271) in the presence of SCF for 48 hours. Proliferation was measured by thymidine incorporation assay. Bars denote the mean thymidine incorporation (cpm ± SEM) of 6 different experiments performed in replicates of 6 (A), and one of the 2 representative experiments performed in replicates of 4 (B). Asterisk indicates P < .05 α4β1 (H296) and α4β1 and α5β1 (CH296) versus α5β1 (CH271).
