Ligation via α₄β₁ induces cell death in G1E-ER2 cells.

G1E-ER2 erythroid progenitors were cultured on FN peptides mediating adhesion to α₄β₁ (H296) or α₅β₁ (CH271) or both α₄β₁ and α₅β₁(CH296) in the absence (A) or presence (B) of SCF for 48 hours. Cell death was quantitated by performing annexin V and PI staining as described in “Materials and methods.” Bars denote the percentage of total cell death (± SD) of 2 independent experiments performed in replicates of 3. Asterisk indicates P < .05 α₄β₁ (H296) and α₄β₁ and α₅β₁(CH296) versus α₅β₁ (CH271).