Analysis showing that Sp17 is expressed in myeloma cells and only in normal testis.

(A) PCR analysis using a pair of sequence-specific primers for Sp17 produced a positive signal of around 500 bp. Lane 1 shows Sp17-positive myeloma bone marrow; lane 2, Sp17-negative myeloma bone marrow; lane 3, normal bone marrow; M, molecular marker. Lane i shows PCR of DNAse I-treated RNA; lane ii, PCR of RNA that had undergone RT; lane iii, control amplification for GAPDH gene segment. (B) Western blot analysis showing the expression of Sp17 protein in myeloma cells and not normal bone marrow cells. Lanes 1 through 6 show lysates from fresh myeloma cells; lane 7, lysate from ARK-B myeloma cell line; lanes 8 and 9, lysates from normal bone marrow. (C) Northern blot and RT-PCR analysis of RNA from a panel of normal tissue. Panel i shows a strong signal, of approximately 1.3 kb, in normal testis and a weak signal in normal prostate detected in Northern blot analysis; panel ii, 28S ribosomal RNA; panel iii, RT-PCR showing Sp17 transcripts in only normal testis and a myeloma tumor cell RNA; panel iv, control amplification for GAPDH gene segment.