Fig. 2.
![Fig. 2. [Ca++]i changes in CCR2- and CCR5-transfected cells. / Murine pre-B cells stably expressing human CCR2 or CCR5 were loaded with FURA-2, and [Ca++]i-dependent fluorescence changes were recorded after sequential stimulation with 1 μM eotaxin and 10 nM MCP-1 (for CCR2-expressing cells, A) or with 1 nM MIP-1β (for CCR5-expressing cells, B). Controls were run under the same conditions, using buffer instead of eotaxin. The tracings are representative of 2 independent experiments with each cell line.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/7/10.1182_blood.v97.7.1920/6/h80710851002.jpeg?Expires=1735576816&Signature=iPvo92-5BGUda9sEUN3G-egkG2LlCh4X27yEoC~8CPDMN-QNgwp2OaTX2YKYzkot6qd3AbtUI0Uy1Kx3BaP-lbh3uIBYcAttThWocm9onCPPFyJpaFVrkLxyvgGihE2QxvosWCmUG9Y-gX-0axcOABhh9XbtZQ14IyPDVoZ81Ux6m2viU4RutnNmqOQY1vsrj93dwkiNhFswZItq4aV67aBpVEA6Ku-CZs~iWf2aRaLME55MGVZDGHX1ojJmU1kY-iaECfkzR~wj9oc9HLzASAUATX1WRXfWkNwrFVLyHRsxkKfakWbp2kLXKl1WRWP8FOAF1JVI7LT1gwcD4Bi4Sg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
[Ca++]i changes in CCR2- and CCR5-transfected cells.
Murine pre-B cells stably expressing human CCR2 or CCR5 were loaded with FURA-2, and [Ca++]i-dependent fluorescence changes were recorded after sequential stimulation with 1 μM eotaxin and 10 nM MCP-1 (for CCR2-expressing cells, A) or with 1 nM MIP-1β (for CCR5-expressing cells, B). Controls were run under the same conditions, using buffer instead of eotaxin. The tracings are representative of 2 independent experiments with each cell line.
