Fig. 6.
CTLA-4 expression on thymic emigrants.
Thymic lobes were cultured as in Figure 2 and beginning on day 12, emigrants were collected for 18, 42, or 66 hours, then analyzed for expression of CTLA-4. For comparison, splenic T cells were activated for 72 hours with different concentrations of anti-CD3, then analyzed for CTLA-4 expression.