Coexpression of recombinant full-length WT and vWF 2A/IIE at different proportions of WT to mutant vWF.

Only the ultralarge multimers (arrows) were absent in the 2A/IIE transfection experiment, especially at the 1:39 proportion of WT to mutant DNA. Relative to recombinant WT vWF, concentrations of the larger multimers were decreased. Intervening bands like those observed in the other mutants were not present.