Fig. 3.
Endothelial surface TNF is in the transmembrane form.
Cytofluorimetric analysis of immortalized HUVECs without (A) or with (B) acid wash was performed as described in the text. FACS analysis was performed using TNF-specific mAb T1 (αTNF, solid line) versus an isotype-matched control antibody (Co, dotted line).