Fig. 6.
Treatment group mouse A39 shows
BCR/ABL transcript levels similar to those of a young preleukemic P190 transgenic mouse. Total RNA was isolated from the bone marrow of BCR/ABL transgenics, including A39, a terminally ill leukemic mouse, and a young healthy mouse. Competitive RT-PCR was performed as described in “Materials and methods.” The numbers below the lanes indicate the number of competitor molecules added to the PCR reaction. The equivalence interval was determined by comparing the intensity of the band resulting from the testerBCR/ABL with the band resulting from the competitor sample. For the leukemic mouse, the interval is between 106 and 106.5, for A39 between 104 and 104.5, and for the preleukemic mouse between 104.5 and 105.
