American Society of Hematology

Fig. 2.

$Fig. 2. Analysis of the progenitor content in different cell fractions purified on the expression of CD34, CD41, and CD42. / CD34+ cells were purified from cord blood and adult mobilized blood and cultured for 6 days in serum-free medium in the presence of SCF (50 ng/mL) and PEG-rHuMGDF (10 ng/mL) and sorted on 6 fractions according to the CD34, CD41, and CD42 expression. Cells were grown in methylcellulose in the presence of PEG-rHuMGDF, SCF, G-CSF, IL-6, IL-3, and Epo for CFU-GM and BFU-E assays, and in fibrin clot in the presence of PEG-rHuMGDF, SCF, IL-6, G-CSF, and Epo for CFU-MK and BFU-E/MK assays. Experiments were performed in triplicate. Results presented are derived from 10 different samples. Cloning efficiency of the CD34+CD41−CD42− cell fraction was quite similar in samples from cord blood and adult blood (219.4/103, 112.7/103, 45.4/103, and 26.3/103 for BFU-E, CFU-GM, CFU-MK, and BFU-E/MK for leukapheresis vs 184.4/103, 107/103, 34.7/103, and 15.6/103 for cord blood). Thus, cloning efficiency in each fraction was referred to that observed in the CD34+CD41−CD42− cell fraction and expressed as a percentage. P was calculated by the student t test: *.01 < P < .05; **P < .01.$

Analysis of the progenitor content in different cell fractions purified on the expression of CD34, CD41, and CD42.

CD34⁺ cells were purified from cord blood and adult mobilized blood and cultured for 6 days in serum-free medium in the presence of SCF (50 ng/mL) and PEG-rHuMGDF (10 ng/mL) and sorted on 6 fractions according to the CD34, CD41, and CD42 expression. Cells were grown in methylcellulose in the presence of PEG-rHuMGDF, SCF, G-CSF, IL-6, IL-3, and Epo for CFU-GM and BFU-E assays, and in fibrin clot in the presence of PEG-rHuMGDF, SCF, IL-6, G-CSF, and Epo for CFU-MK and BFU-E/MK assays. Experiments were performed in triplicate. Results presented are derived from 10 different samples. Cloning efficiency of the CD34⁺CD41⁻CD42⁻ cell fraction was quite similar in samples from cord blood and adult blood (219.4/10³, 112.7/10³, 45.4/10³, and 26.3/10³ for BFU-E, CFU-GM, CFU-MK, and BFU-E/MK for leukapheresis vs 184.4/10³, 107/10³, 34.7/10³, and 15.6/10³ for cord blood). Thus, cloning efficiency in each fraction was referred to that observed in the CD34⁺CD41⁻CD42⁻ cell fraction and expressed as a percentage. P was calculated by the student t test: *.01 < P < .05; **P < .01.

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