Analysis of the cell composition of individual clones derived from the CD34⁺CD41⁻ and CD34⁺CD41⁺CD42⁻ cells derived from cord blood at day 11 of culture.

The results presented are those obtained from 4 independent experiments that have been pooled, representing analysis of 112 and 95 clones, respectively. Culture conditions were serum-free plus a combination of cytokines (PEG-rHuMGDF, SCF, Epo, G-CSF, IL-3, and IL-6). Only clones having more than 200 cells were analyzed on the expression of CD15, CD41, and GPA in triple-staining experiments by means of flow cytometry. Each cell type was identified on the basis of phenotypic and scatter properties.