Assessment of eGFP expression in leukocytes in a macaque following reinfusion of transduced CD34⁺ cells.

Flow cytometric detection of eGFP in peripheral blood leukocytes in an untransplanted animal (A) and in animals having transplantation with CD34⁺ bone marrow cells transduced with LZRS/eGFP (B). eGFP⁺ cells are shown in granulocytes (Mm 280-97) and CD20⁺ B cells (Mm 278-97) 3 weeks after transplantation (B). CD3⁺ T cells from Mm 280-97 at 5 weeks after transplantation were analyzed to demonstrate eGFP expression in CD3⁺CD4⁺ cells as well as CD3⁺CD4⁻ cells, 93% of which were CD8⁺. Gates for expression of eGFP were established using an untransplanted animal as a control at each time point examined. The percentage of gated cells in each quadrant is indicated.